Skip to main content
. 2021 Feb 19;14(4):101039. doi: 10.1016/j.tranon.2021.101039

Fig. 5.

Fig. 5

NSE required β-catenin to promote migration, invasion, and EMT of SCLC cells. (A-D) Western blotting and qRT-PCR assay for the expression of β-catenin, slug, E-cadherin, and N-cadherin in NSE-overexpressing H446 cells in the presence of shβ-catenin (A, B) and in NSE-silencing H69 cells in the presence of β-catenin (C, D). (E) Wound healing assay for the migration of NSE-overexpressing H446 cells in the presence of shβ-catenin (Scale bar: 100 um). (F, G) Transwell assay for the invasion and migration of NSE-overexpressing H446 cells which were further infected with virus expressing shβ-catenin (F) and NSE-silencing H69 cells which were further infected with virus expressing β-catenin (G) (Scale bar: 50 um). Data are presented as mean values ± SD of three independent experiments. *p < 0.05 using the two-sided Student's t-test. ns: no significance.