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. 2021 Feb 22;45(4):39. doi: 10.3892/or.2021.7990

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Both H19 and p53 share complementary binding sites with miR-675. (A) Binding sites between miR-675 and p53 were identified. A dual-luciferase reporter assay was subsequently conducted to verify the relationship between miR-675 and p53. (B) H19 and miR-675 expression levels in HaCaT cells transfected with pcDNA3.1-H19 were significantly upregulated compared with the pcDNA3.1-NC group. (C) H19 and miR-675 expression levels in SCL1 and A431 cells transfected with H19-siRNA were significantly downregulated compared with the H19-NC group. (D) Binding sites between miR-675 and p53 were identified. A dual-luciferase reporter assay was conducted to verify the relationship between miR-675 and p53. **P<0.01 and ***P<0.001. miR, microRNA; NC, negative control; siRNA, small interfering RNA; WT, wild-type; Mut, mutant.