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. 2021 Feb 25;12:147. doi: 10.1186/s13287-021-02215-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Characterization of control-MSCs and IPF-MSCs. a Surface markers of control-MSCs and IPF-MSCs were evaluated by flow cytometry. Both control-MSCs and IPF-MSCs expressed CD73, CD90, and CD105 but not CD34 or CD45. b Adipogenic differentiation evaluated by Oil Red staining and quantification of adipogenic efficiency in control-MSCs and IPF-MSCs. c Osteogenic differentiation determined by Alizarin red staining and quantification of osteogenic efficiency in control-MSCs and IPF-MSCs. d Chondrogenic differentiation determined by Alcian blue staining and quantification of chondrogenic efficiency in control-MSCs and IPF-MSCs. All data were obtained from at least three independent experiments and each error bar represents the mean ± SEM. Scale bar = 200 μm. ***p < 0 .001