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. 2021 Feb 25;12:147. doi: 10.1186/s13287-021-02215-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — miR-199a-5p induced cellular senescence of MSCs. a Expression of miR-199a-5p measured in serum from control donors and patients. b Expression of miR-199a-5p measured in control-MSCs and IPF-MSCs. c Western blotting analysis of p21 and p16 protein expression in control-MSCs treated with miR control or miR-199a- 5p mimic. d Representative images and quantitative analysis of SA-β-gal staining in control-MSCs transfected with miR control or miR-199a-5p mimic. Scale bar = 200 μm. e Western blotting analysis of p21 and p16 protein expression in IPF-MSCs treated with miR control or miR-199a- 5p inhibitor. f Representative images and quantitative analysis of SA-β-gal staining in IPF-MSCs transfected with miR control or miR-199a-5p inhibitor. Scale bar = 200 μm. All data were obtained from at least three independent experiments and each error bar represents the mean ± SEM. **p < 0.01; ***p < 0.001