. 2021 Feb 24;52:31. doi: 10.1186/s13567-020-00881-y

Table 7.

Details of immunological diagnosis in possum.

Assay test	N/E	n_Se + n_Sp	Antigens	Se (%)	Sp (%)	References
ELISA	N	29 + 100	M. bovis culture filtrate MBP70	45^a 21^a 28^b	96^a 98^a 99^b	[100]
RT	N	38 + 91	ESAT-6, CFP-10, MPB83	44.7	85.7	[109]

Assay test

N/E

n_Se + n_Sp

Antigens

Se (%)

Sp (%)

References

ELISA

29 + 100

M. bovis culture filtrate MBP70

45^a

21^a

28^b

96^a

98^a

99^b

[100]

38 + 91

ESAT-6, CFP-10, MPB83

44.7

85.7

[109]

N/E: natural or experimental infection; n_Se: number of TB positive animals used for evaluation of Se; n_Sp: number of TB negative animals used for evaluation of Sp; Se: sensitivity; Sp: specificity; ELISA: enzyme linked immunosorbent assay; RT: lateral flow rapid test (name not mentioned- Chembio Diagnostic Systems, Inc., USA).

^a Se or Sp of ELISA where blocking antibody against MBP70 is not used.

^b Se or Sp of blocking ELISA where a monoclonal antibody against MPB70 is used.