Fig. 2.

The effects of circMMP11 inhibition on proliferation, migration, invasion, and apoptosis of breast cancer cells. a, b The expression of circMMP11 was measured by RT-qPCR assay in MCF-7 and MDA-MB-231 cells transfected with si-NC, si-circMMP11#1, si-circMMP11#2, or si-circMMP11#3. c–j MCF-7 and MDA-MB-231 cells were transfected with si-NC or si-circMMP11#2. c–e The proliferation ability of MCF-7 and MDA-MB-231 cells were estimated by MTT and colony-forming assays. f, g Transwell assay was conducted to assess migration and invasion of MCF-7 and MDA-MB-231 cells. h The apoptotic cells were monitored by flow cytometry assay. i, j The protein expression levels of E-cadherin and Vimentin were quantified by western blot assay. **P < 0.01, ***P < 0.001