Figure 4.

Effect of cambinol on protein expression levels in RPMI8226 cells. Western blotting was performed to assess the protein expression levels in RPMI8226 cells following 48 h treatment with 0, 40, 80 and 160 µM cambinol. (A) SIRT1 activity was detected using a SIRT1 Assay kit. (B) Expression levels of PARP and pro-caspase-3. (C) Expression levels of the Ac-p53, p53, Bcl-2, CyclinD1 and p21. β-actin expression was used as a loading control for each panel. *P<0.05, **P<0.01 and ***P<0.001. Ac-p53, Acetylated p53; SIRT1, silent mating type information regulation 2 homolog 1; PARP, poly(ADP-ribose) polymerase 1.