Fig. 3.

UTRN inhibited proliferation via p38 and JNK1/c-Jun pathways in A375 melanoma cell. a–c qRT-PCR (a) and WB (b, c) results showed UTRN expression after stable transduction with lentiviral vectors. d CCK-8 assay of A375 cell line including EXP, EXP-NC and Blank control groups. e, f EdU incorporation assay in A375‑EXP‑UTRN transfected cells and negative controls. Magnification, × 200. g, h WB results showed that p38, JNK and c-Jun were down-regulated in EXP group. i After treatment with 8-pCPT-2′-O-Me-cAMP (100 μM, 30 min), the levels of p38, JNK and c-Jun were enhanced. j The proliferative ability was increased after 8-pCPT-2′-O-Me-cAMP treatment. 8-CPT: 8-pCPT-2′-O-Me-cAMP. Data was analyzed by the Student’s t-test. Bars represent mean ± SD; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001