Fig. 1.

AVT inhibits c-Maf transcriptional activity. a HEK293T cells expressing c-Maf/Otub1/MARE.Luci were treated with natural products from TargetMol® or vehicle for 24 h, followed by luciferase assay. b RPMI-8226 cells were treated with various candidates for 24 h, followed by cell lysate preparations and WB assays.CTR, vehicle control; 1, flubendazole; 12, AVT; 21, vincristine sulfate; 23, Ouabain. c RPMI-8226 cells were transfected with plasmids as indicated for 48 h. Cells were then re-plated and treated with 0.1% DMSO or AVT for 24 h before being harvested for luciferase activity measurements. d-e, MM cells were treated with AVT for 24 h, followed by WB (d) or RT-PCR (e) assays to evaluate the expression of specific genes