Fig. 3.

AVT accumulates c-Maf polyubiquitination by disrupting the interaction between Otub1 and c-Maf. a c-Maf and Otub1 plasmids were co-transfected into HEK293T cells for 12 h followed by AVT treatment for 24 h. Cell lysates were subjected to a co-IP/WB assay. b RPMI-8226 and LP1 cells were treated by AVT for 24 h before being lysed and subjected to co-IP/WB assays. c c-Maf and Otub1 plasmids were co-transfected into HEK293T cells for 12 h, followed by AVT treatment. Cell lysates were subjected to a co-IP/WB assay. d RPMI-8226 and LP1 cells were treated with AVT for 24 h before being subjected to co-IP/WB assays. e–f. c-Maf and pMARE.Luci plasmids were co-transfected into HEK293T cells with or without Otub1 (e) or Otub1 siRNA (f), followed by AVT treatment. Cell lysates were subjected to luciferase and WB assays