Fig. 5.

Otub1 expression partly abolishes AVT-induced MM cell apoptosis. a MM cells were infected with lentiviral Otub1 for 96 h, followed by AVT treated for 24 h. Cell lysates were then prepared for WB assays against indicated proteins. b The cell lysates from a were subjected to co-IP/WB as indicated. c RPMI-8226 and LP1 cells were treated as a before being stained with Annexin V-FITC/PI staining and flow cytometric analyses. d MM cells were treated with increased AVT for 24 h, followed by WB assays against indicated proteins