Figure 4.

IGF-1 is a target gene of miR-130a-3p. (A) Based on TargetScan, a conserved binding site was identified in the 3′UTR of IGF-1, a well-known oncogenic gene in thyroid cancer. (B) Dual luciferase reporter assay demonstrated that miR-130a-3p significantly suppressed the relative luciferase activity of pmirGLO-IGF-1-3′UTR. (C) The serum IGF-1 level was significantly increased in patients with DTC compared with patients wit benign thyroid tumors and healthy controls. (D) Pearson's correlation analysis indicated that serum miR-130a-3p was negatively correlated with exosomal miR-130a-3p. ***P<0.001. DTC, differentiated thyroid cancer; IGF-1, insulin-like growth factor; UTR, untranslated region.