Fig. 3.

Abl∆IDR does not rescue embryonic morphogenesis. a–f Cuticle preparations. Anterior up. a Wildtype, ventral side right, revealing the segmental array of denticle belts and naked cuticle. Arrowhead: head involution was completed and there is a well-formed head skeleton. Arrow. Germband retraction was completed, positioning the spiracles at the posterior end. Scale bar = 50 µm. b–d Examples of cuticles from progeny of Abl∆IDR; abl⁴/Df mothers crossed to abl⁴/+ fathers. b Least severe phenotype. Head involution, dorsal closure (arrowhead) and germband retraction (arrow) failed. c Intermediate phenotype, with large hole in the ventral cuticle. d Severe phenotype. Only fragments of cuticle remain. f Range of cuticle defects seen in the progeny of females whose germlines are homozygous for abl⁴ crossed to abl⁴/+ fathers, carrying the transgenes indicated in G maternally and zygotically. Arrows and arrowheads as in a–d. Images in a and f are from Rogers et al., 2016, where we developed this cuticle scoring scheme. g Abl∆IDR; ablM/Z embryos have more frequent defects in epithelial integriy than either Abl∆CR1; ablM/Z embryos or even than unrescued ablM/Z embryos. Frequencies of each phenotype in the indicated genotypes. Statistical test used was Fisher’s Exact Test