Table 1.
Comparison of AblΔIDR rescue of adult and embryonic viability with other Abl constructs
| Construct | Residues changed or deleted | Percent rescue of adult viability in abl4/Df(3L)st-j7 (normalized to WT)* | Probability that viability is higher than adults with no transgene | Probability that rescue is better or worse than that by Abl WT | Probability that rescue is better or worse than that by Abl ΔCR1 | Percent rescue of progeny embryonic viabilty of abl4/Df(3L)st-j7 X abl4/+** | Probability that rescue is worse than that by Abl WT | Probability that rescue is better or worse than that by Abl ΔCR1 | Percent rescue of progeny embryonic viabilty of abl4 maternal germ line clone X abl4/+*** | Probability that rescue is worse than that of Abl WT | Probability that rescue is better or worse than that of ablMZ | Probability that rescue is better or worse than that by Abl ΔCR1 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No Transgene | N/A | 20.4 (n = 145)# | N/A | p < 0.0001 | p = 0.4474 | N/A | N/A | N/A | 8.2% (n = 527)# | p < 0.0001 | n/a | p = 0.0246 |
| Abl WT | N/A | 100% (n = 386)# | p < 0.0001 | N/A | p ≤ 0.0001 | 89.4 (n = 901)# | N/A | p < 0.0001 | 38.9% (n = 540)# | n/a | p < 0.0001 | p < 0.0001 |
| Abl∆IDR | 679–1398 | 48.1% (n = 223) | p = 0.0075 | p < 0.0001 | p = 0.3064 | 26.2% (n = 378) | p < 0.0001 | p < 0.0001 | 1.1% (n = 569) | p < 0.0001 | p < 0.0001 | p < 0.0001 |
| AblΔFABD | 1418–1520 | 140% (n = 173)# | p < 0.0001 | p < 0.0001 | p < 0.0001 | 97.1 (n = 362)# | p < 0.0001 | p < 0.0001 | 34.6% (n = 515)# | p = 0.16 | p < 0.0001 | p < 0.0001 |
| AblKDΔFABD | K417N 1418–1520 | 81.5% (n = 585)# | p < 0.0001 | p = 0.0108 | p = 0.0029 | 52.1 (n = 259)# | p < 0.0001 | p = 0.7268 | 28.2 (n = 560)# | p = 0.0002 | p < 0.0001 | p < 0.0001 |
| AblΔCR1 | 734–789 | 30.7% (n = 48)# | p = 0.4474 | p < 0.0001 | N/A | 53.9 (n = 267)# | p < 0.0001 | N/A | 12.4% (n = 606)# | p < 0.0001 | p = 0.02 | N/A |
Comparison of AblΔIDR rescue of adult and embryonic viability in Abl null mutant background with other Abl constructs with statistical significance. A two-tailed Fisher's Exact Test was used to determine p-values of whether there was a statistically significant difference in extent of rescue between genotypes (see Methods)
#Data from Rogers et al. 2016 for comparison; n = number of adults/embryos scored; N/A = not applicable; italics = rescue better than indicated genotype; bold italics = rescue worse than indicated genotype
*Graphed in Fig. 1d
**Graphed in Fig. 1e
***Graphed in Fig. 1f