Fig. 6.

MIR4435-2HG links glycolysis and its related signal pathways including the epithelial-to-mesenchymal transition (EMT) and immune regulation. a Cumulative distribution curve (CDF) of glycolysis-gene correlations with or without adjustment for MIR4453-2HG by using a first-order partial correlation. Solid lines indicate CDFs of correlation coefficients between glycolysis scores and gene expressions without adjustment. Dashed lines indicate first-order partial correlation-adjusted relations between glycolysis scores and gene expressions. These two distributions were compared using the Kolmogorov–Smirnov test. The x-axis represents Pearson correlation coefficients between glycolysis scores and gene expressions, and the y-axis represents cumulative probabilities. b A circular bar plot displaying MIR4435-2HG-associated signal pathways. Genes significantly associated with MIR4435-2HG but independent of the methylation status and copy number alterations were identified using a multivariate linear regression. Pathways enriched in MIR4435-2HG-associated genes are shown in the circular bar plot. The height of the bars indicates the degree of enrichment. Only pathways with a false detection rate (FDR) of < 0.01 are included in the plot. c A volcano plot indicating MIR4453-2HG-associated genes that were independent of copy number alterations and the DNA methylation status. The x-axis represents multivariate linear regression-adjusted coefficients between MIR-4435-2HG and glycolysis scores. Gene candidates involved in immune-related signal pathways are labeled with a black round line, and their names are indicated in the plots