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. 2021 Feb 17;21(4):300. doi: 10.3892/ol.2021.12561

Figure 4.

Figure 4.

JAG1 is a direct target of miR-153. (A) Predicted binding sites between JAG1 and miR-153-3p (shown in red print). (B) Relative luciferase activity in SW1990 cells co-transfected with miR-153 mimic or miR-NC and JAG1-WT, JAG1-MT or JAG1-3′UTR (the JAG1-3′UTR contains the whole length of 3′UTR). *P<0.05, **P<0.01 compared with the blank vector control. (C) Reverse transcription-quantitative PCR analysis of JAG1 mRNA expression levels in stably transduced cells. ***P<0.001 with comparisons shown by lines. (D) Western blot quantification of JAG1 protein expression levels in stably transduced cells. (E) Pearson's correlation analysis of the expression levels (log2) between miR-153 and JAG1 in pancreatic ductal adenocarcinoma tissues. JAG1, jagged canonical Notch ligand 1; miR-153, microRNA-153; NC, negative control; WT, wild-type; MT, mutant; UTR, untranslated region; Inhi, inhibitor.