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. 2021 Feb 25;52:33. doi: 10.1186/s13567-021-00909-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Reversal of epithelial polarity in piglet colon organoid. After 7 days of culture in Matrigel™ (A), piglet colon organoids were cultured in suspension for 24 h (B). Organoids were observed by confocal laser scanning microscopy to visualize horizontal (xy, A1 and B1) and vertical (xz, A2 and B2) sections. Phalloidin staining (red) shows actin and DAPI staining (blue) shows nuclei. Arrows indicate the apical side of epithelial cells. L Lumen.