Table 3.

Chicken intestinal organoids

Segment	Application	Segment	Crypt isolation	Growth medium	Reference
Caecum	Model development	Caecum	Collagenase, 37 °C, 1.5 h	50% (DMEM + 10% FBS), 50% L-WRN CM, 10 µM SB431542, 10 µM Y27632	[14]
Jejunum	Model development	Jejunum	2 mM EDTA, 4 °C, 3 × 30 min	DMEM/F12, 10 mM HEPES, 2 mM glutaMAX, 50 ng/mL EGF, 100 ng/mL Noggin, 500 ng/mL R-spondin1	[40]
Small intestine	Chemical treatments	Small intestine	Mechanical	DMEM/F12, 10% FBS, insulin-transferrin-selenium, polyamine, bovine pituitary extract	[61]
Embryonic Small intestine	Model development, TLR agonist and Lactobacillus acidophilus treatment	Embryonic Small intestine	2.5 mM EGTA, 0.5% glucose, 4 °C, 15 min + 45 min twice	DMEM/F12, Glutamax, insulin-transferrin-selenium premix, 25 ng/mL EGF, 25 ng/mL rh Noggin,250 ng/mL rh R-spondin1, 5 µg/mL Prostaglandin E2	[15, 31, 41, 42, 62]

CM: conditioned medium, L-WRN: L cell line engineered to secrete Wnt3a, R-spondin 3 and Noggin, rh: recombinant human, FBS: fetal bovine serum, EGF: epidermal growth factor. Antibiotics and antifungals added in the growth medium are not presented.