Figure 4.
Pyrosequencing verification of the β-values of the TRIM58 (A) and ZN132 (B) genes based on the Infinium assay. Since tissue samples from the present 162 patients were exhausted after the Infinium assay, pyrosequencing and the Infinium assay were performed using 26 paired samples of adjacent normal lung tissue and their corresponding cancerous tissues in another cohort [validation cohort (n = 52)] of lung adenocarcinomas. The β-values of cg20855565 and cg13877915 for the TRIM58 and ZNF132 genes and the DNA methylation levels in the promoter regions based on pyrosequencing were significantly correlated with each other in the validation cohort (Pearson’s correlation coefficient r = −0.922 and 0.900, and P = 2.234 × 10−11 and 3.939 × 10−10, respectively), indicating that the Infinium data had been successfully verified using pyrosequencing.