Table 1.

Immunohistochemical staining of RAD9, DNMT1 and DNMT3B proteins in non-cancer human prostate tissues

Sample number	Non-cancer prostate tissue	RAD9 staininga	DNMT1 staininga	DNMT3B staininga
1	141N	−	−	−
2	142N	−/+, partial +	−/+	−/+
3	143N	−/+, partial +	−	−
4	320N	−	−	−
5	321N	−	−	−
6	322N	−	−/+	−/+
7	323N	−	−	−
8	N1	−	−	−
9	N2	−	−	−
10	N3	−	−	−
11	N4	−	−	−
12	N5	−/+, partial +	−	+/++
13	N6	−, partial −/+	−/+	−/+
14	N7	−	−, partial −/+	−
15	N8	−	−	−
16	N9	−	−/+	−, partial +
17	N10	−	−	+
18	N11	−/+	−	−
19	N12	−	−	−/+
20	N13	−/+	−	+
21	N14	−	−	−/+
22	N15	−	−	−
	Percent positiveb	13.6 (3/22)	0 (0/22)	18.8 (4/22)

^aImmunostaining for RAD9, DNMT1 and DNMT3B proteins designated ‘—’ (negative), ‘+’ (weak), ‘++’ (strong), ‘−/+’ (borderline weakly positive) or ‘+/++’ (borderline weak to strongly positive). When ‘partial’ is indicated, the first designation is the predominant staining intensity and only a small portion of the tissue stains with the second indication of intensity.

^bBorderline weakly positive (−/+) is considered negative for the Percent Positive calculation.