KEY RESOURCES TABLE.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse monoclonal anti-alpha Tubulin (DM1A) | Thermo Fisher Scientific | Cat#62204; RRID: AB_1965960 |
| Rabbit monoclonal anti-GAPDH (14C10) | Cell Signaling Technology | Cat#2118 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 2-Vinylpyridine | Sigma-Aldrich | Cat#132292; CAS: 100–69-6 |
| 5-Sulfosalicylic acid dihydrate | Sigma-Aldrich | Cat#S2130; CAS: 5965–83-3 |
| 5,5’-Dithiobis(2-nitrobenzoic acid) | Sigma-Aldrich | Cat#D8130; CAS: 69–78-3 |
| CellTracker Red CMTPX Dye | Thermo Fisher Scientific | Cat#C34552 |
| Ethylenediaminetetraacetic acid disodium salt dihydrate | Sigma-Aldrich | Cat#E5134; CAS: 6381–92-6 |
| Glucose oxidase from Aspergillus niger | Sigma-Aldrich | Cat#G7141 CAS: 9001–37-0 |
| Glutathione Reductase from baker’s yeast (S. cerevisiae) | Sigma-Aldrich | Cat#G3664; CAS: 9001–48-3 |
| L-Glutathione oxidized | Sigma-Aldrich | Cat#G6654; CAS: 27025–41-8 |
| L-Glutathione reduced | Sigma-Aldrich | Cat#S4251; CAS: 70–18-8 |
| N-TER Nanoparticle siRNA Transfection System | Sigma-Aldrich | Cat#N2913 |
| Potassium phosphate dibasic | Sigma-Aldrich | Cat#P8281; CAS: 7758–11-4 |
| Potassium phosphate monobasic | Sigma-Aldrich | Cat#P5655; CAS: 7778–77-0 |
| Triethanolamine | Sigma-Aldrich | Cat#T58300; CAS: 102–71-6 |
| Triton X-100 | Sigma-Aldrich | Cat#X100; CAS: 9002–93-1 |
| β-Nicotinamide adenine dinucleotide 2’-phosphate reduced tetrasodium salt hydrate | Sigma-Aldrich | Cat#N7505; CAS: 2646–71-1 (anhydrous) |
| Critical Commercial Assays | ||
| CellTiter-Glo 2.0 Cell Viability Assay | Promega | Cat#G9241 |
| Deposited Data | ||
| Enzyme turnover numbers | BRENDA database | https://www.brenda-enzymes.org |
| FBA models of TCGA tumors | This paper | https://github.com/kemplab/FBA-pipeline |
| Mutational effect predictions | Envision database | https://envision.gs.washington.edu/shiny/envision_new/ |
| TCGA clinical data | The Cancer Genome Atlas; Synapse TCGA_Pancancer project | https://www.synapse.org/#!Synapse:syn300013/wiki/27406 |
| TCGA genomic data | The Cancer Genome Atlas; GDC data portal | https://portal.gdc.cancer.gov |
| TCGA transcriptomic data | The Cancer Genome Atlas; (Rahman et al., 2015) | GEO: GSE62944 |
| Thermodynamic parameters | Virtual Metabolic Human database | https://www.vmh.life |
| Experimental Models: Cell Lines | ||
| Human: MDA-MB-231 NQO1(-) | Dr. David Boothman, Indiana University (Huang et al., 2016) | Derived from CVCL_0062 |
| Human: MDA-MB-231 NQO1(+) | Dr. David Boothman, Indiana University (Huang et al., 2016) | Derived from CVCL_0062 |
| Human: M059J | ATCC | CRL-2366 |
| Human: M059K | ATCC | CRL-2365 |
| Human: rSCC-61 | Dr. Cristina Furdui, Wake Forest University (Bansal et al., 2014) | N/A |
| Human: SCC-61 | Dr. Jeffrey N. Myers, UT MD Anderson Cancer Center | CVCL_7118 |
| Oligonucleotides | ||
| siRNA panel – See Table S7 | Sigma-Aldrich | N/A |
| siRNA: MISSION siRNA Universal Negative Control #1 | Sigma-Aldrich | Cat#SIC001 |
| Software and Algorithms | ||
| Source data and original code | This paper | https://github.com/kemplab/FBA-pipeline |
| Optimization software | Gurobi 8.0 | https://www.gurobi.com |