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. 2021 Jan 10;13(3):3886–3897. doi: 10.18632/aging.202356

Figure 5.

Figure 5

Praja2 inhibited the progression of GC cells through KSR1-MEK-ERK axis. Praja2 or NC was transfected into MKN-45 cells, and treated with MG132 or FGF2 (MEK-ERK activator). (A) CCK8 for viability of MKN-45 cells. (B) Edu assay for proliferation of MKN-45 cells. Scale bar, 40 μm. (C) Wound healing assay for migration of MKN-45 cells. Scale bar, 60 μm. (D) Transwell assay for invasion of MKN-45 cells. Scale bar, 60 μm. (E) Flow cytometry assay for apoptosis of MKN-45 cells. Data are mean ± SD; *P < 0.05.