Table 3. DNA methylation rate of sequences in En1, Otx1, and Nkx2-1 in 12 weeks and 19 months mice between SCA3/MJD transgenic and WT groups.

Gene	Sequence	Time	Group	Levels of DNA methylation	p value
En1	S1 (En1_2)	3 month	SCA3/MJD	0.222 ± 0.082	0.033*
			control	0.067 ± 0.017
		19 month	SCA3/MJD	0.172 ± 0.009	0.286
			control	0.128 ± 0.054
	S2 (En1_3)	3 month	SCA3/MJD	0.504 ± 0.160	0.784
			control	0.459 ± 0.210
		19 month	SCA3/MJD	0.364 ± 0.081	0.608
			control	0.307 ± 0.192
Otx1	S3 (Otx1)	3 month	SCA3/MJD	0.324 ± 0.043	0.005*
			control	0.185 ± 0.009
		19 month	SCA3/MJD	0.164 ± 0.145	0.474
			control	0.248 ± 0.116
Nkx2-1	S4 (Nkx2-1_2)	3 month	SCA3/MJD	0.063 ± 0.023	0.009*
			control	0.163 ± 0.029
		19 month	SCA3/MJD	0.167 ± 0.093	0.239
			control	0.270 ± 0.09
	S5 (Nkx2-1_3)	3 month	SCA3/MJD	0.656 ± 0.116	0.192
			control	0.527 ± 0.015
		19 month	SCA3/MJD	0.622 ± 0.223	0.745