| Gene |
primer nucleotide sequence |
| UBE2C |
Forward: 5’-GCAGTCGTGTTCTCCGAGTT-3’ |
|
Reverse: 5’-GCTCCTGCTGTAGCCTTTTG-3’ |
| LYPLA2 |
Forward: 5’-AAGAAGGCAGCAGAGAACATC-3’ |
|
Reverse: 5’-CTCCCAGGACGATTCGATTG-3’ |
| PPIF |
Forward: 5’- TGGTGACACAGGCCACAGAC-3’ |
|
Reverse: 5’- CCGGAGCACAGGAGCTTACA-3’ |
| BIRC5 |
Forward: 5’-GACGACCCCATGCAAAGGAA-3’ |
|
Reverse: 5’- GTGGCACCAGGGAATAAACC-3’ |
| ABT1 |
Forward: 5’-ACGGGTAGTGCCAGGTATTG-3’ |
|
Reverse: 5’-CGGTCCTCAGCCTGAAAGAA-3’ |
| PRRC2A |
Forward: 5’-GCCACAGGGATTCCCAATCA-3’ |
|
Reverse: 5’-TTGGGGGAGTTGCCCTTTTT-3’ |
| TICRR |
Forward: 5’- CACGGGAGACGAAGAGGT-3’ |
|
Reverse: 5’- CTGGAACAGCAGCGGAGA-3’ |
| ASCC1 |
Forward: 5’-CTCACCACGACGAGGACCG-3’ |
|
Reverse: 5’-TCCAATTATGCCCGTGAGGG-3’ |
| WT1 |
Forward: 5’-CCAAATGACATCCCAGCTTG-3’ |
|
Reverse: 5’-GTGTGGTTATCGCTCTCGTAC-3’ |
| SOWAHC |
Forward: 5’-CTGGTCAAGCGGGACTTCAT-3’ |
|
Reverse: 5’-CTCCGACTCAGGTACTGGGA-3’ |
| E-cadherin |
Forward: 5′-CGAGAGCTACACGTTCACGG-3′ |
|
Reverse: 5′-GGGTGTCGAGGGAAAAATAGG-3′ |
| N-cadherin |
Forward: 5′-TGCGGTACAGTGTAACTGGG-3′ |
|
Reverse: 5′-GAAACCGGGCTATCTGCTCG-3′ |
| Vimentin |
Forward: 5′-TGCCGTTGAAGCTGCTAACTA-3′ |
|
Reverse: 5′-CCAGAGGGAGTGAATCCAGATTA-3′ |
| Snail |
Forward: 5′-ACTGCAACAAGGAATACCTCAG-3′ |
|
Reverse: 5′-GCACTGGTACTTCTTGACATCTG-3′ |
| Twist |
Forward: 5′-ATTCAAAGAAACAGGGCGTGG-3′ |
|
Reverse: 5′-CCTTTCAGTGGCTGATTGGC-3′ |
| MMP9 |
Forward: 5′-TTGACAGCGACAAGAAGTGG-3′ |
|
Reverse: 5′-GCCATTCACGTCGTCCTTAT-3′ |
| MMP2 |
Forward: 5′-TCTCCTGACATTGACCTTGGC-3′ |
|
Reverse: 5′-CAAGGTGCTGGCTGAGTAGATC-3′ |
| CCND1 |
Forward:5’-AAACAGATCATCCGCAAACAC-3’ |
|
Reverse:5’-GTTGGGGCTCCTCAGGTTC-3’ |
| MYC |
Forward:5’-CCTGGTGCTCCATGAGGAGA-3’ |
|
Reverse:5’-TCCAGCAGAAGGTGATCCAGAC-3’ |
| GAPDH |
Forward: 5’-ACCCAGAAGACTGTGGATGG-3’ |
|
Reverse: 5’-TCAGCTCAGGGATGACCTTG-3’ |
