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. 2020 Dec 3;13(3):3353–3367. doi: 10.18632/aging.202184

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Copyright: © 2020 Hu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Effect of AA supplementation on the developmental competence of in vitro aged porcine oocytes after parthenogenetic activation. (A) Blastocyst formation at day 7 in each group (Left); and representative fluorescence images of blastocyst staining with Hoechst 33342 in each group (Right). Scale bar=100 μm. (B) Percentage of 2-cell embryos, (C) 4-cell embryos and (D) blastocyst formation in each group. (E) Total number of cells in blastocysts from each group. (F) Diameter of blastocysts from each group. The number of embryos examined in each group is shown by the bars. Statistically significant differences are indicated by different letters (p<0.05).