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. Author manuscript; available in PMC: 2021 Feb 25.
Published in final edited form as: Methods Mol Biol. 2016;1354:39–47. doi: 10.1007/978-1-4939-3046-3_3

Fig. 2.

Fig. 2

PF74 and CPSF6 (1–321) increase the stability of in vitro-assembled HIV-1 CA-NC complexes, whereas the CAI peptide decreases the stability. In contrast, the CAI peptide with a randomized sequence (CAIctrl) did not show an effect on the stability of HIV-1 CA-NC complexes. The stability of HIV-1 CA-NC complexes in destabilization buffer (stabilization assay) (a) or destabilization buffer in cell extracts (destabilization assay) (b) supplemented with 10 μM PF74, 50 μM CAI, or CAIctrl peptide was measured. The assay without cellular extract was performed additionally using 10 μg of the protein CPSF6 (amino acids 1–321).