Table 2. List of designed primers to determine four genes.
Primers | Forward (5’→3’) | Reverse (5’→3’) |
---|---|---|
SIRT1 | AGGAGCAGATTAGTAGGCGGCTT | AGCTCTCTCTGGAACATCAGGCT |
FZD6 | TGCTGTCTTCTGGGTTGGAAGC | GCTCCTGTGCTGGTTCCCAT |
THBS4 | CATGGTGCAGGGTGTTGGGA | GGCCCACAGCGGTAAGATCC |
CPNE3 | GGCTCCAATGGTGACCCAAGG | TGAGGAGGTATCTGAGCGCCAA |
miR-451a | AAACCGTTACCATTACTGAGTT | GTGCAGGGTCCGAGGTATTC |
miR-142-3p | CTCCTGTAGTGTTTCCTAC | GACTGTTCCTCTCTTCCTC |
U6 | CTCGCTTCGGCAGCACA | AACGCTTCACGAATTTGCGT |