Fig 2. PCR analysis of mixtures of parasite cells and human HepG2 cells.

A constant number of 10⁶ human HepG2 cells has been mixed with 10² (lanes 1), 10³ (lanes 2), 10⁴ (lanes 3), 10⁵ (lanes 4), 10⁶ (lanes 5) and no (lanes 6) parasite cells prior to DNA extraction and PCR. Analyses in (A) have been performed with primer-set 1 (expected size 374 bp), those in (B) with primer-set 2 (expected size 493 bp). Exp1-3 indicates the results of three independently performed experiments. PCR amplificates have been separated on a 1.5% agarose gel and stained with ethidium bromide. DNA marker sizes (in bp) are indicated to the right of the marker lane.