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. Author manuscript; available in PMC: 2021 Feb 25.
Published in final edited form as: Cell Rep. 2020 Dec 1;33(9):108418. doi: 10.1016/j.celrep.2020.108418

Figure 5. SUMO ligase activity of TRIM11, and functional cooperation of TRIM11’s discrete activities in the clearance of Atxn1 82Q.

Figure 5.

(A and B) SUMOylation of HA-Atxn1 82Q by Flag-TRIM proteins in the presence of 6xHis-SUMO2 in HEK293T cells.

(C) SUMOylation of Flag-Atxn1 82Q by HA-TRIM11 in HEK293T cells treated with control or SUMO2/3 siRNA.

(D) SUMOylation of Flag-Atxnl 82Q, but not α-Syn, by HA-TRIM11 in the presence of 6xHis-SUMO2 in HEK293T cells stably expressing α-Syn.

(E) Levels of GFP-Atxn1 82Q when expressed alone or together with Flag-TRIM11/TRIM112EA in HCT116 cells.

(F) Amyloid contents in HCT116 cells transfected with TRIM11 or TRIM112EA

(G) Half-life of Flag-Atxn1 82Q in HEK293T cells when co-expressed with the indicated TRIM11 proteins. Relative Atxn1 82Q/GAPDH ratios are shown. The exposure of different blots was adjusted so that band intensities at time 0 were comparable.

Data are mean ± SD (n = 4 biological replicates). **P < 0.01.