KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Anti-TRIM11 | Millipore | Cat# ABC926; RRID: AB_2861378 |
| Anti-α-synuclein | Cell Signaling | Cat# 2642; RRID: AB_2192679 |
| Anti-α-synuclein phopho-Ser129 | Abcam | Cat# ab168381; RRID: AB_2728613 |
| Anti-α-synuclein phopho-Ser129 | Biolegend | Cat# 825701; RRID: AB_2564891 |
| Anti-SUMO2/3 | Abgent | Cat# AP1224a; RRID: AB_2198423 |
| Anti-GFP mouse mAb | Clontech | Cat# 632380; RRID: AB_10013427 |
| Anti-GFP rabbit pAb | Invitrogen | Cat#: A11122; RRID:AB_221569 |
| Anti-HA rabbit pAb | Santa Cruz | Cat# sc-805; RRID: AB_631618 |
| Anti-HA rabbit mAb (C29F4) | Cell Signaling | Cat# 3724; RRID: AB_1549585 |
| Anti-HA Affinity Matrix Rat mAb | Sigma-Aldrich | Cat# 11815016001; RRID: AB_390914 |
| Anti-GAPDH | Santa Cruz | Cat# sc-32233; RRID: AB_627679 |
| Anti-GST | GE Healthcare Life Sciences | Cat# 27457701; RRID: AB_771432 |
| Anti-β-actin | Sigma-Aldrich | Cat# A5441; RRID: AB_476744 |
| Anti-α-tubulin | Sigma-Aldrich | Cat# T9026; RRID: AB_477953 |
| Anti-Flag rabbit mAb | Cell signaling | Cat# 14793; RRID: AB_2572291 |
| Anti-Flag mouse mAb (M2) | Sigma-Aldrich | Cat# F1804; RRID: AB_262044 |
| Anti-FLAG M2 agarose beads | Sigma-Aldrich | Cat# A1205, RRID: AB_2687448 |
| Anti-Hsp70 | Enzo Life Sciences | Cat# ADI-SPA-810-D; RRID: AB_2039260 |
| Anti-Hsp90 | Cell Signaling | Cat# 4874; RRID: AB_2121214 |
| Anti-NeuN | Millipore | Cat# MAB377; RRID: AB_2298772 |
| Anti-Tyrosine Hydroxylase | Millipore | Cat# 657012; RRID:AB_696697 |
| Bacterial and Virus Strains | ||
| BL21(DE3) | ThermoFisher | Cat# C600003 |
| DH5α Competent Cells | ThermoFisher | Cat# 18258012 |
| TRIM11 lentiviral vector | This paper | N/A |
| TRIM11 2EA lentiviral vector | This paper | N/A |
| mCherry-TRIM11 lentiviral vector | (Chen et al., 2017) | N/A |
| mCherry lentiviral vector | (Chen et al., 2017) | N/A |
| α-Syn Lentiviral vector | This paper | N/A |
| TRIM11 AAV9 vector | This paper | N/A |
| GFP AAV9 vector | This paper | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| SUMO E1 | Boston Biochem | Cat# E-315 |
| SUMO E2 (UbcH9) | Boston Biochem | Cat# E2-465 |
| 6xHis-SUMO2 | Boston Biochem | Cat# UL-753 |
| Human α-synuclein | The Michael J. Fox Foundation | N/A |
| Human α-synuclein S87N | (Luna et al., 2018) | N/A |
| Mouse α-synuclein | (Luna et al., 2018) | N/A |
| GFP | This paper | N/A |
| GST | This paper | N/A |
| GST-TRIM11 | This paper | N/A |
| GST-TRIM11 2EA | This paper | N/A |
| 6xHis-TRIM11 | This paper | N/A |
| 6xHis-TRIM11 ∆R | This paper | N/A |
| 6xHis-TRIM11 ∆B | This paper | N/A |
| 6xHis-TRIM11 ∆CC | This paper | N/A |
| 6xHis-TRIM11 N | This paper | N/A |
| 6xHis-TRIM11 C | This paper | N/A |
| 6xHis-TRIM11 M6 | This paper | N/A |
| 6xHis-TRIM11 LA | This paper | N/A |
| Flag-Atxn1 82Q-HA | This paper | N/A |
| GST-TRIM21 | This paper | N/A |
| Flag-PML (isoform VI) | This paper | N/A |
| Hsp40 | Enzo Life Sciences | Cat# ADI-SPP-400 |
| Hsp70 | Enzo Life Sciences | Cat# ADI-NSP-555 |
| Hsp104 | (Jackrel et al., 2014) | N/A |
| Hsp104 A503S | (Jackrel et al., 2014) | N/A |
| Luciferase | Sigma-Aldrich | Cat# L9506 |
| Citrate Synthase | Sigma-Aldrich | Cat# C3260 |
| Mg2+-ATP | Sigma-Aldrich | Cat# A9187 |
| Phosphocreatine | Sigma-Aldrich | Cat# P1937 |
| Creatine kinase | Sigma-Aldrich | Cat# C3755 |
| FLAG Peptide | Sigma-Aldrich | Cat# F3290 |
| MG132 | Sigma-Aldrich | Cat# C2211 |
| Benzonase | Sigma-Aldrich | Cat# E1014 |
| TEV protease | Sigma-Aldrich | Cat# T4455 |
| Isopropyl-1-thio-D-galactopyranoside (IPTG) | Sigma-Aldrich | Cat# I6758 |
| Complete protease inhibitor Cocktail | Sigma-Aldrich | Cat# 11697498001 |
| L-Glutathione reduced | Sigma-Aldrich | Cat# G4251 |
| Ni-NTA Agarose | Qiagen | Cat# 30230 |
| Glutathione Superflow Agarose | Thermo Fisher | Cat# 25236 |
| TRIzol Reagent | Invitrogen | Cat# 15596 |
| Amylose resin | New England BioLabs | Cat# E8021 |
| 4’,6-diamidino-2-phenylindole (DAPI) | Vector Laboratories | H-1200 |
| Critical Commercial Assays | ||
| ATP Affinity Test Kit | Jena Bioscience GmbH | Cat# AK-102 |
| MTT assay | Sigma-Aldrich | Cat# M2128 |
| Phusion® High-Fidelity PCR Kit | NEB | Cat# E0553 |
| First-strand cDNA Synthesis System | Marligen Biosciences | Cat# 11801 |
| CellTiter-Glo Luminescent Cell Viability Assay | Promega | Cat# G7570 |
| Super Sensitive™ IHC Detection System | BioGenex | Cat# QD400 |
| Experimental Models: Cell Lines | ||
| HCT116 | ATCC | Cat# CCL-247; RRID: CVCL_0291 |
| A549 | ATCC | Cat# CCL-185; RRID: CVCL_0023 |
| SH-SY5Y | ATCC | Cat# CRL-2266; RRID: CVCL_0019 |
| HeLa | ATCC | Cat# CCL-2; RRID: CVCL_0030 |
| HEK293T | ATCC | Cat# CRL-11268; RRID: CVCL_0063 |
| QBI-293 | QBiogene | N/A |
| Mouse primary neurons | Penn Neuron Culture Service Center and our own lab | N/A |
| Experimental Models: Organisms/Strains | ||
| Mouse: male C3H/HeJ | Jackson Laboratories | Stock# 000659 |
| Oligonucleotides | ||
| siRNAs target mouse TRIM11 | Santa Cruz | Cat# sc-76735 |
| siRNA target human TRIM19 | Santa Cruz | Cat# sc-36284 |
| siRNAs target Human TRIM11 | IDT | Table S1 |
| Negative control siRNA | IDT | Table S1 |
| RT-PCR primers for human and/or mouse TRIM11, Hsp70, Hsp90, and GAPDH | IDT | Table S1 |
| Recombinant DNA | ||
| pcDNA3.1-Flag-TRIM11 | This paper | N/A |
| pcDNA3.1-Flag-TRIM11 ∆R | This paper | N/A |
| pcDNA3.1-Flag-TRIM11 ∆B | This paper | N/A |
| pcDNA3.1-Flag-TRIM11 ∆CC | This paper | N/A |
| pcDNA3.1-Flag-TRIM11 N | This paper | N/A |
| pcDNA3.1-Flag-TRIM11 C | This paper | N/A |
| pcDNA3.1-Flag-TRIM11 M6 | This paper | N/A |
| pcDNA3.1-Flag-TRIM11 2EA | This paper | N/A |
| pcDNA3.1-Flag-TRIM11 LA | This paper | N/A |
| pEGFP-C1-TRIM11 | This paper | N/A |
| pEGFP-C1-Flag-TRIM11 | This paper | N/A |
| pEGFP-C1-TRIM11 LA | This paper | N/A |
| pEGFP-C1-Hsp70 | This paper | N/A |
| pRK5-Flag-TRIM19 (VI) | (Guo et al., 2014) | N/A |
| pRK5-mCherry-TRIM19 (VI) | This paper | N/A |
| pRK5-Flag-TRIM21 | This paper | N/A |
| pRK5-His-SUMO2 | (Guo et al., 2014) | N/A |
| pRK5-GFP-Atxn1 82Q | (Guo et al., 2014) | N/A |
| pRK5-HA-Atxn1 82Q-Flag | (Guo et al., 2014) | N/A |
| pRK5-Flag-Atxn1 82Q | (Guo et al., 2014) | N/A |
| pRK5-Flag-Atxn1 30Q | (Guo et al., 2014) | N/A |
| pRK5-Flag-Luciferase | (Guo et al., 2014) | N/A |
| pRK5-Flag-NLS-Luciferase | (Guo et al., 2014) | N/A |
| pTRPE | (Posey et al., 2016) | N/A |
| pTRPE-TRIM11 | This paper | N/A |
| pTRPE-TRIM11 2EA | This paper | N/A |
| pTRPE-α-synuclein | This paper | N/A |
| pTRPE-GFP-Atxn1 82Q | This paper | N/A |
| pTRPE-mCherry | (Chen et al., 2017) | N/A |
| pTRPE-mCherry-TRIM11 | (Chen et al., 2017) | N/A |
| AAV9-GFP | Penn Gene Therapy Vector Core | N/A |
| AAV9-TRIM11 | Penn Gene Therapy Vector Core | N/A |
| pFastBac-GST-TRIM11 | This paper | N/A |
| pGEX-1ZT-TRIM11 | This paper | N/A |
| pGEX-1ZT-TRIM11 2EA | This paper | N/A |
| pMAL-c2X-TRIM11-6xHis | This paper | N/A |
| pMAL-c2X-TRIM11 ∆R-6xHis | This paper | N/A |
| pMAL-c2X-TRIM11 ∆B-6xHis | This paper | N/A |
| pMAL-c2X-TRIM11 ∆CC-6xHis | This paper | N/A |
| pMAL-c2X-TRIM11 N-6xHis | This paper | N/A |
| pMAL-c2X-TRIM11 C-6xHis | This paper | N/A |
| pMAL-c2X-TRIM11 M6-6xHis | This paper | N/A |
| pMAL-c2X-TRIM11 LA-6xHis | This paper | N/A |
| pTAT-HA | Gift from Steven Dowdy | Addgene Plasmid Cat# 35612 |
| pTAT-HA-TRIM11 | This paper | N/A |
| Luciferase-6xHis | (Sharma et al., 2010) | N/A |
| pPROEX HTb-6xHis-TEV-GFP | (Jackrel and Shorter, 2014) | N/A |
| Software and Algorithms | ||
| GraphPad Prism 7 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| ImageJ | ImageJ.Ink | https://imagej.net/Welcome |
| In Cell Developer Toolbox 1.9.2 software | GE Healthcare | N/A |
| ZEN lite | Carl Zeiss Microscopy | N/A |