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. 2021 Feb 18;10:e66288. doi: 10.7554/eLife.66288

Figure 7. Trim71 represses mRNA translation at post-initiation step(s) in mouse embryonic stem cells (mESCs).

Figure 7.

(A) FLuc reporters for the tethering assay. (B) The FLuc activity and mRNA level determined in the tethering assay. (C) The IRES-containing bicistronic reporters. (D) mRNA levels from the IRES-containing reporters. (E) Luciferase activities from the IRES-containing reporters. (F) The poly(A) minus FLuc reporters. (G) mRNA levels from the poly(A) minus reporters. (H) FLuc activities from the poly(A) minus reporters. The results represent the means (± SD) of three independent experiments. *p<0.05, and ns. not significant (p>0.05) by the Student’s t-test.