Table 5.
Fold increase of PATRR DNA compared to backgrounds 6q26, 9q34 and 10q26.1 for the hESC-derived spermatogenic cells
| 6q26 | 9q34 | 10q26.1 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| γH2AX | Rad51 | NBS1 | γH2AX | Rad51 | NBS1 | γH2AX | Rad51 | NBS1 | |
| PATRR17 | 1.30 | 1.26 | 1.36 | 1.29 | 1.13 | 1.20 | 1.27 | 1.06 | 1.04 |
| PATRR8 | 1.06 | 1.06 | 1.21 | 1.01 | 0.98 | 0.95 | 1.07 | 1.07 | 0.87 |
| PATRR11 | 1.45 | 1.16 | 1.21 | 1.37 | 1.06 | 1.07 | 1.32 | 1.07 | 1.18 |
| PATRR22 | 1.46 | 1.39 | 1.19 | 1.69 | 1.50 | 1.18 | 1.43 | 1.54 | 1.97 |
| PATRR Median | 1.23 | 1.18 | 1.22 | 1.22 | 1.08 | 1.07 | 1.26 | 1.15 | 1.04 |
| PATRR Mean | 1.43 | 1.25 | 1.38 | 1.40 | 1.25 | 1.20 | 1.44 | 1.32 | 1.18 |
| P-value | 0.0011 | 0.0005 | 0.0039 | 0.0001 | 0.0172 | 0.0044 | 0.0007 | 0.0287 | 0.0283 |
Data from ChIP assays using DSB-associated antibodies γH2AX, Rad51 and NBS1. Median values are reported for individual PATRRs. Data from all four PATRRs are combined to generate the total PATRR median and mean values for a given antibody. P-values are calculated for the total PATRR value for each antibody using the one-sample t-test comparing the total PATRR to the mean value of 1 using GraphPad Software at www.graphpad.com.