Figure 9.

Baseline phenotypic differences in adult and fetal cardiac fibroblasts Adult and fetal cardiac fibroblasts were cultured in DMEM with 10% FBS and 1% antibiotic-antimycotic until 80% confluent. The fibroblasts were then serum starved in DMEM with 1% FBS and 1% antibiotic-antimycotic for 12 h before being harvested for total RNA expression. QPCR demonstrates that adult cardiac fibroblasts have significantly higher expression of Col1a1 (A), Col3a1 (B), TIMP1 (C), and MMP9 (D) than fetal cardiac fibroblasts at baseline. *p < 0.05.