Fig. 1.
Nr3c1 and Nr3c2 mRNA and protein levels in the hippocampus ofEmx1-Nr3c1−/−mice.(A) GR/Nr3c1 mRNA levels measured by RT-qPCR (n = 6 per group; two-way ANOVA genotype: F1,20 = 47.01, ****p < 0.0001). (B) Western blot analyses of GR and (C) relative quantities of GR in female and male controls (n = 3 per group). (D) MR/Nr3c2 mRNA levels measured by RT-qPCR (n = 6 per group; two-way). (E) Western blot analyses of MR. (F, left) Relative quantities of MR in female Emx1-Nr3c1−/− compared to female controls (n = 3 per group, two-tailed Welch's t-test: t2.036 = 4.558, *p = 0.0434). (F, right) Relative quantities of MR in male Emx1-Nr3c1−/− compared to male controls (n = 3 per group, two-tailed Welch's t-test: t2.4 = 1.837, p = 0.1861). β-actin was used as normalizer across both RT-qPCR and Western blot.