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. 2021 Jan 15;40(8):1516–1530. doi: 10.1038/s41388-020-01627-y

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© The Author(s), under exclusive licence to Springer Nature Limited part of Springer Nature 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A ELISA detection of MDSCs-associated cytokines in U251-Ctrl and U251-BATF2 tissues (n = 3, independent experiment, *p < 0.05, **p < 0.01, ***p < 0.001). B ELISA detection of SDF-1α in 7, 14, and 21 days in U251-Ctrl and U251-BATF2 tumour (n = 3, independent experiment, *p < 0.05, **p < 0.01, ***p < 0.001). C Representative IHC images of BATF2 and SDF-1α on consecutive sections of U251-Ctrl and U251-BATF2 intracranial tumours. Scale bars, 100 μm. D Representative images of immunofluorescent staining with SDF-1α (green), SV40 large T (red) and DAPI (blue) in U251-Ctrl and U251-BATF2 intracranial tumours. Scale bars, 25 μm. E Percentages of CXCR4 and CXCR7 expressing cells in CD11b⁺Gr1⁺ MDSCs isolated from tumours by FACs analysis. F Representative image of U251 tumour injected with U251-Ctrl- and U251-BATF2-derived EVs for four times, the subcutaneous tumour volume and SDF-1α detection by ELISA (n = 3 independent experiment, *p < 0.05, **p < 0.01, ***p < 0.001). G Western blot detection of HIF-1α and SDF-1α in 7, 14 and 21 days during U251 tumour progression. H EVs labelled by PKH67 added to phalloidin staining U251 cells and western blot detection of HIF-1α and SDF-1α. Scale bars, 20 μm. I ELISA and qPCR detection of SDF-1α in U251 tumour treated with Ctrl-EVs and BATF2-EVs (n = 3, independent experiment, *p < 0.05, **p < 0.01, ***p < 0.001). J ELISA detection of SDF-1α with and without 1% Triton X-100 under hypoxia condition (n = 3, independent experiment, *p < 0.05, **p < 0.01, ***p < 0.001). K Nano-FCM analysis and statistics of SDF-1α expression in Ctrl-EVs and BATF2-EVs (n = 3, independent experiment, *p < 0.05, **p < 0.01, ***p < 0.001).