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. 2021 Jan 8;40(8):1440–1457. doi: 10.1038/s41388-020-01626-z

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© The Author(s), under exclusive licence to Springer Nature Limited part of Springer Nature 2021, corrected publication 2024

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Fig. 8 — A IF staining was performed to detected the level of E-cadherin (red) and vimentin (green) after PAN02 treatment with the indicated concentrations of N4 for 24 h. DAPI (blue) was used to stain Nuclei. Scale bar, 20 μm. B Western blot assays were used to examine the expression of E-cadherin, N-cadherin and vimentin after PAN02 treatment with N4 for 24 h. C IHC of vimentin, F4/80 and α-SMA in tumor tissue from orthotopic models. Data represents the mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s. not significant by one-way ANOVA followed by multiple-comparison tests. D Quantification of vimentin, F4/80 and α-SMA in pancreatic tumors from PDAC. Scale bar, 50 μm.