Extended Data Fig. 1. Capturing SARS-CoV-2 RNAs and bound proteins with RAP-MS.
a, Alignment of protein-crosslinked RNA fragments to the SARS-CoV-2 genome following RNA antisense purification of SARS-CoV-2 RNAs from infected Huh7 cells. Two replicate experiments are shown. b, Fraction of crosslinked RNA fragments mapping to the human or SARS-CoV-2 genomes in pilot RAP-MS experiments. c, Correlation plot for two replicate RAP experiments. CPM values for SARS-CoV-2 genes are shown. CPM: counts per million. d, As in b, but for full-scale SARS-CoV-2 RNA RAP-MS and RMRP RAP-MS experiments. e, Western blot of two replicate SARS-CoV-2 RNA and RMRP RAP-MS experiments. Indicated antibodies were used for protein detection.