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. 2021 Jan 25;6(3):354–365. doi: 10.1038/s41564-020-00840-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, Map of eight Northern California groundwater sites sampled in this study (image from Google Maps). Insets: geological maps (i)–(iii) show the sampled areas (black boxes). J, marine sedimentary and metasedimentary rocks (Jurassic); KJf_m, marine sedimentary and metasedimentary rocks (Cretaceous–Jurassic); Q, marine and non-marine (continental) sedimentary rocks (Pleistocene–Holocene); Qv, marine sedimentary and metasedimentary rocks (Cretaceous–Jurassic); um, plutonic (Mesozoic); K, marine sedimentary rocks (Pliocene); Kl, marine sedimentary and metasedimentary rocks (Lower Cretaceous); Ep, marine sedimentary rocks (Paleocene); E, marine sedimentary rocks (Eocene); QPc, non-marine (continental) sedimentary rocks (Pliocene–Pleistocene); Tv, volcanic rocks (Tertiary). Scale bars, 23.3 km (large map), 3.2 km (i and ii) and 9.7 km (iii). b, Phylum-level breakdown (with the exception of CPR and DPANN superphyla) of rpS3 genes detected in each site. The sampling dates for each site are indicated. c, Rank abundance curves showing the 30 rpS3 genes with highest relative coverage identified for each site. The hatched bars indicate an unbinned rpS3 gene.