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. Author manuscript; available in PMC: 2021 Jul 11.
Published in final edited form as: Nat Immunol. 2021 Jan 11;22(3):347–357. doi: 10.1038/s41590-020-00847-4

Figure 1. γδ2 T cells are activated in P. falciparum infection.

Figure 1.

a, Frequency of circulating lymphocytes from 8 healthy donors (HD) and 8 P. falciparum patients (Pf). CD3+CD4+ (CD4), CD3+CD8+ (CD8), CD3+TCRδ2+ (γδ2), CD3−CD56+ (NK). b,c, Circulating γδ2 T cell activation in 8 HD and 5 Pf was evaluated by CD69 and CD16 staining. d, Intracellular granulysin (GNLY), perforin (PFN) and granzyme B (GzmB) in 8 HD and 8 Pf. e, CFSE-stained iRBC were co-cultured with fresh purified γδ2 T cells from 3 HD and 3 Pf at indicated E:T ratios for 12 hr and iRBC lysis was assessed by flow cytometry analysis of the number of viable CFSE+ cells relative to added counting beads. f, Plasma GNLY concentration in 16 HD and 22 Pf patients by GNLY ELISA. Statistical analysis was performed by two-tailed nonparametric unpaired t-test (a,f), two-way ANOVA with Sidak’s multiple comparisons test (d), and area under the curve, followed by two-tailed nonparametric paired t-test, followed by two-tailed nonparametric paired t-test (e). Mean ± s.e.m. is shown. P value: *<0.05, **<0.01, ***<0.001, ****<0.0001.