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. Author manuscript; available in PMC: 2021 Jul 11.
Published in final edited form as: Nat Immunol. 2021 Jan 11;22(3):347–357. doi: 10.1038/s41590-020-00847-4

Extended Data 6. iRBC lysis and parasite killing at different stages of infection by purified cytotoxic granule proteins.

Extended Data 6

a, Electron microscopy of a ring stage iRBC treated or not with GzmB, PFN and GNLY showing disruption of morphology after treatment (right). In (a), parasitophorous vacuole (PV) detachment is indicated by a black arrow and increased parasite vacuolization by a red arrow. Parasite nucleus (N), hemoglobin vacuole (Hb), hemozoin (H). b, Imaging flow cytometry gating strategy for parasite developmental stages based on DNA content by Hoechst staining. M merozoites, R rings, T trophozoites, S schizonts. c, GzmB-AF488 uptake in the presence or absence of GNLY. Scale bar: 500 nm (a). n, biological independent samples. Data shown are representative of at least three independent experiments.