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. Author manuscript; available in PMC: 2021 Jul 11.
Published in final edited form as: Nat Immunol. 2021 Jan 11;22(3):347–357. doi: 10.1038/s41590-020-00847-4

Figure 5. P. falciparum HMBPP activates γδ2 T cells via the γδTCR and BTN3A1.

Figure 5.

a,b, Highly purified HD γδ2 T cells, pre-activated with IL-2 and IL-15, were co-cultured with iRBCs in the presence of indicated blocking or isotype control (Iso) antibodies (n=4) and assayed for γδ2 T cell degranulation by cell surface CD107a (a) and RBC lysis (b) by flow cytometry. c,d, HD γδ2 T cells were co-cultured with iRBCs pre-treated with indicated combinations of fosmidomycin and IPP, uRBCs or HMBPP to assess degranulation (n=3) (c) and RBC lysis (n=5) (d). e,f, γδ2 T cell or iRBCs were pre-incubated with blocking antibodies to γδTCR, BTN3A1 or isotype control (Iso) before co-culture (n=3) to assess degranulation (e) and RBC lysis (f). n, biological independent samples. Statistical analysis was performed by one-way ANOVA with Tukey’s multiple comparisons test. Mean ± s.e.m. is shown; P value: *<0.05, **<0.01, ***<0.001, ****<0.0001. Data shown are representative of three independent experiments.