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. Author manuscript; available in PMC: 2021 Jul 11.
Published in final edited form as: Nat Immunol. 2021 Jan 11;22(3):347–357. doi: 10.1038/s41590-020-00847-4

Extended Data 1. Gating strategy for phenotypic analysis of peripheral blood lymphocytes from healthy donors (HD) and P. falciparum-infected patients (Pf).

Extended Data 1

a, PBMCs were stained with the Live/Dead viability dye and antibodies to CD3, CD4, CD8, TCRδ2 and CD56. Single live cells were gated on SSC-A vs FSC-A and dead cells were excluded. Lymphocyte subpopulations were gated as: CD3+CD4+ (CD4+ T cells), CD3+CD8+ (CD8+ T cells), CD3+TCRδ2+ (γδ2 T cells) and CD3−CD56+ (NK). b, PBMCs co-stained for CD69, CD16 and cytotoxic effector protein expression (GNLY, PFN and GzmB) were gated on γδ2 T cells.