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. 2020 Sep 30;70(3):803–815. doi: 10.1007/s00262-020-02727-0

Table 2.

Primer sequences for qRT-PCR

Primer Sequence
ARG1

Forward, 5′- GTGGAAACTTGCATGGACAAC -3′

Reverse, 5′- AATCCTGGCACATCGGGAATC -3′

IDO

Forward, 5′- GCCAGCTTCGAGAAAGAGTTG -3′

Reverse, 5′- ATCCCAGAACTAGACGTGCAA -3′

IL1B

Forward, 5′- ATGATGGCTTATTACAGTGGCAA -3′

Reverse, 5′- GTCGGAGATTCGTAGCTGGA -3′

IL6

Forward, 5′- ACTCACCTCTTCAGAACGAATTG -3’

Reverse, 5′- CCATCTTTGGAAGGTTCAGGTTG -3′

CCR2

Forward, 5′- CCACATCTCGTTCTCGGTTTATC -3′

Reverse, 5′- CAGGGAGCACCGTAATCATAATC-3′

CD274

Forward, 5′- TGGCATTTGCTGAACGCATTT -3′

Reverse, 5′- TGCAGCCAGGTCTAATTGTTTT -3′

LGALS9

Forward, 5′- GGGCGCAGACAAAAACCTC -3′

Reverse, 5′- GGAGTAGAGAACATCTGTCCAGG -3′

β-actin

Forward, 5′- AGAGCTACGAGCTGCCTGAC -3′

Reverse, 5′- AGCACTGTGTTGGCGTACAG -3′