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. Author manuscript; available in PMC: 2021 Jul 28.
Published in final edited form as: Nat Immunol. 2021 Jan 28;22(3):312–321. doi: 10.1038/s41590-020-00859-0

Extended Data Fig. 10. Deletion of ATG5, ATG7, and BECLIN1 does not induce type I IFN.

Extended Data Fig. 10

BMDMs from mice with myeloid-specific deficiency (LysM-Cre-targeted deletion) of Atg7 (a-b), Atg5, (c-d), and Beclin1 (e-f) were treated as shown and analyzed for expression of Ifnb1, Ifit1, and the respective deleted gene targets. Results are a combination of BMDM cultures from two animals (n=6), except for (b) where n=3. CCCP = carbonyl cyanide m-chlorophenyl hydrazine. g-i, Lungs and spleen from naïve mice from the three strains were harvested and analyzed by qPCR for the targets shown. n=6, 58 week-old females for (g), n=4 for LysMCre−Atg5Fx/Fx lungs, n=5 for LysMCre−Atg5Fx/Fx spleen and n=6 for LysMCre+Atg5Fx/Fx lungs and spleens, 9-14 week-old females for (h), and N=3, 9-14 week-old females for (i). Data are mean +/− s.e.m, ns = not significant. Two-tailed unpaired t-test.