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. Author manuscript; available in PMC: 2021 Jul 28.
Published in final edited form as: Nat Immunol. 2021 Jan 28;22(3):312–321. doi: 10.1038/s41590-020-00859-0

Figure 3: Type I interferon induction in Irgm1-null fibroblasts is cGAS/STING-dependent.

Figure 3:

a,b, Interferon-β (Ifnb1) and interferon-stimulated gene (Ifit1) expression in murine embryonic fibroblasts (MEFs) of indicated genotypes, treated with or without IFN-γ (16h) (n=3). c, Ifnb1 expression in MEFs co-treated with brefeldin A (2 μg/ml) and IFN-γ (n=3). d, Phosphorylation of TBK1 (S172), total TBK1, and β-actin (loading control) detected by Western blot in cells untreated or treated with IFN-γ. At right, densitometry of four independent experiments is shown. e, Ifnb1 expression in MEFs pretreated with TBK1 inhibitor (MRT-67307, 10 μM, 2h) and then IFN-γ (16h) (n=3). f, Ifnb1 and Ifit1 expression in MEFs transfected with two different siRNAs against Irf3 or control siRNA and then treated as shown (n=3). b, d, and e are representative of three independent experiments. a, c, and f are representative of two independent experiments. Data are mean +/− s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001 (Two-tailed unpaired t-test).