Fig.5 ∣. BACH2 deficiency impairs the differentiation of stem-like CD8⁺ T cells.

a, Mixed bone marrow chimeric mice reconstituted with wild-type CD45.2 and wild-type CD45.1 (WT+WT) or Bach2^−/− CD45.2 and wild-type CD45.1 bone marrows (WT+KO) were infected with LCMV clone 13 and analyzed on day 10 p.i.. Frequencies of stem-like (TCF-1^hiTIM-3^lo) cells within H-2D^b GP33 tetramer⁺ CD45.1 and CD45.2 CD8⁺ T cells in chimeras are shown in FACS plots (left) and summary bar graph (right, n=5). b-g, Cas9; P14 CD8⁺ T cells transduced with control or Bach2 gRNA constructs were adoptively transferred into C57BL/6 mice that were subsequently infected with LCMV clone 13. Splenic P14 cells were analyzed on day 7 p.i.. b, Frequencies of stem-like (TCF-1^hiTIM-3^lo) cells within control or Bach2 gRNA transduced P14 CD8⁺ T cells are shown in FACS plots (left) and summary bar graph (right, n=6). c, Numbers of control or Bach2 gRNA transduced stem-like (TCF-1^hiTIM-3^lo, left panel) or terminally exhausted (TCF-1^loTIM-3^hi, right panel) P14 CD8⁺ T cells (n=6). d-g, FACS analyses of Ly108 (d, n=5), PD-1 (e, n=5), TIM-3 (f, n=5), and EOMES (g, n=5) expression in control and Bach2 gRNA transduced stem-like P14 CD8⁺ T cells on day 7 p.i.. n equals the number of mice per group. Each circle represents one mouse. Bar graphs represent mean±s.d.. Data are representative of at least two independent experiments. In a, statistical significance was determined with a two-sided paired Student’s t-test. In b-g, statistical significance was determined with a two-sided unpaired Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.