Extended Data Figure 2. BACH2 overexpression enhances stem-like CD8⁺ T cell differentiation during chronic LCMV infection.

a, Representative FACS plots of TCF-1 and TIM-3 expression in pMIG and BACH2 OE P14 CD8⁺ T cells on day 28 p.i. b, Left panel: representative FACS plots of pMIG and BACH2 OE P14 cells in CD8⁺ T cells on day 14 and day 28 p.i.. Right panel: fold changes in the numbers of BACH2 OE P14 cells relative to the numbers of pMIG P14 cells on day 14 and day 28 p.i.. n=5 mice/group. c-e, FACS analyses of PD-1 (c, n=5 mice/group), TIM-3 (d, n=5 mice/group), TIGIT (e, n=5 mice/group) expression in pMIG and BACH2 OE P14 CD8⁺ T cells on day 14 p.i.. f-h, FACS analyses of PD-1 (f, n=5 mice/group), TIM-3 (g, n=5 mice/group), TIGIT (h, n=5 mice/group) expression in pMIG and BACH2 OE P14 CD8⁺ T cells on day 28 p.i.. i,j, FACS analyses of KLRG1 expression in pMIG and BACH2 OE P14 CD8⁺ T cells on day 14 (i, n=5 mice/group) and day 28 (j, n=5 mice/group) p.i.. k,l, FACS analyses of EOMES expression in pMIG and BACH2 OE P14 CD8⁺ T cells on day 14 (k, n=5 mice/group) and day 28 (l, n=5 mice/group) p.i.. m-o, FACS analyses of granzyme B (m, n=5 mice/group), IFNγ (n, n=5 mice/group), TNFα (o, n=5 mice/group) expression in pMIG and BACH2 OE P14 CD8⁺ T cells on day 7 p.i.. p, Numbers of pMIG (n=4 mice) and BACH2 OE (n=5 mice) P14 CD8⁺ T cells in the liver (left) and the ratio of P14 cells in the liver versus P14 cells in the spleen in pMIG (n=4 mice) and BACH2 OE (n=5 mice) groups (right) on day 7 p.i.. q, Numbers of pMIG (n=4 mice) and BACH2 OE (n=5 mice) P14 CD8⁺ T cells in the lung (left) and the ratio of P14 cells in the lung versus P14 cells in spleen in pMIG (n=4 mice) and BACH2 OE (n=5 mice) groups (right) on day 7 p.i.. r,s, Representative FACS plots (left) and bar graph (right) showing the percentage of stem-like (TCF-1^hiTIM-3^lo) pMIG (n=4 mice) and BACH2 OE (n=5 mice) P14 CD8⁺ T cells in the liver (r) and lung (s). Data are representative of at least two independent experiments. Circles represent individual mice. Bar graphs represent mean±s.d.. Statistical significance was calculated with a two-sided Student’s t-test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001