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. 2021 Feb 12;11:599947. doi: 10.3389/fimmu.2020.599947

Figure 1.

Figure 1

S100A8 and S100A9 are up-regulated in psoriasis like skin inflammation. Naïve BALB/c mice were treated with imiquimod (IMQ) or placebo cream for 7 consecutive days. (A) Typical skin appearance of IMQ- or placebo cream treated BALB/c mice (n = 5 per group). (B) Quantitative measurements of epidermal thickness are shown as means ± SEM. Student’s t-test ***p < 0.001. (C) Representative paraffin skin sections stained with anti-S100A8, anti-S100A9, IgG control and HE. One representative image for each condition is shown (original magnification 10 x, scale bar = 100 µm). (D) Serum concentrations of S100A8/S100A9 in mice was analyzed via ELISA after 7 days of IMQ treatment (▪) and controls (▼) (Student’s t-test **p < 0.01). (E) Differential expression pattern of S100A8, S100A9 and various cytokines on transcriptional level in IMQ (▪)- and placebo (▼)- treated skin sections. Transcripts were measured by quantitative real-time PCR and normalized to RPL housekeeping gene. Values are expressed as mean ± SEM (n ≥ 3 mice per group; Student’s t-test *p < 0.05).