Figure 4.

Expression of S100A8 and S100A9 in naïve primary keratinocytes of wildtype and S100A9^−/− mice. Primary keratinocytes were isolated from wildtype C57BL/6 (WT)- and S100A9^−/− mice and matured for indicated time points ex vivo. (A) Representative immunofluorescence images of cell nuclei (DAPI, blue), S100A8, S100A9, or IgG control (Alexa Fluor 546, red) in primary WT- and S100A9^−/− keratinocytes at indicated stages of maturation (original magnification 20 x, scale bar = 100 µm). (B) Transcriptional expression patterns of S100A8 and S100A9 were determined by quantitative real-time PCR analysis in primary WT (white column)- and S100A9^−/− (black column) keratinocytes at indicated stages of maturation. Columns represent the means ± SEM of at least eight independent experiments. Values are normalized to RPL housekeeping gene. Student’s t-test showed no significances.