Table 2.

Human and mouse colonoid medias.

Colonoid type	Media	Basal Media		Growth factors	Additives
Mouse	rENR	Advanced DMEM/F12 Hepes, GlutaMAX Pen/Strep N-2, B-27 N-Acetylcysteine		Human R-spondin1 Mouse Noggin Human EGF
Mouse	rWENR	Advanced DMEM/F12 Hepes, GlutaMAX Pen/Strep N-2, B-27 N-Acetylcysteine		Human R-spondin1 Mouse Noggin Human EGF Mouse Wnt3a
Mouse	Wnt3a CM + rENR	Advanced DMEM/F12 Hepes, GlutaMAX Pen/Strep N-2, B-27 N-Acetylcysteine		Human R-spondin1 Mouse Noggin Human EGF Mouse Wnt3a
Mouse /Human	WNR CM	Advanced DMEM/F12 20% FBS GlutaMAX Pen/Strep		WNR CM Mouse R-Spondin 3 Mouse Noggin Mouse Wnt3a	TGF-βR Inhibitor ROCK inhibitor
Human	rENR + Wnt3a CM	Advanced DMEM/F12 Hepes, GlutaMAX Pen/Strep N-2, B-27 N-Acetylcysteine	Advanced DMEM/F12 20% FBS GlutaMAX Pen/Strep	Human R-spondin1 Mouse Noggin Human EGF Wnt3a CM	Gastrin Nicotinamide TGF-βR Inhibitor, P38 inhibitor ROCK inhibitor
Human	rWENR	Advanced DMEM/F12 Hepes, GlutaMAX Pen/Strep N-2, B-27 N-Acetylcysteine		Human R-spondin1 Mouse Noggin Human EGF Mouse Wnt3a	Gastrin Nicotinamide TGF-βR Inhibitor, P38 inhibitor ROCK inhibitor